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Fig. 6. TPA increases the amount of activated PKC-ß associated with RACK-I. Subconfluent melanocyte cultures were treated with 10–7 M TPA or vehicle alone for 90 minutes. Equal amounts of proteins from vehicle- and TPA-treated cell lysates were subjected to immunoprecipitation using an antibody against RACK-I. The immunoprecipitated proteins were separated and subjected to immunoblot analysis as described for Fig. 5. Densitometric analysis of each band was performed. When the ratio of PKC-ß to RACK-I in vehicle-treated samples was set arbitrarily at 1.0, the ratio in TPA-treated samples was 2.67.





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