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Fig. 3. Chromosomes in klp67A mutant primary spermatocytes are abnormally dynamic and undergo exaggerated periods of reorientation during prometaphase. (A) Time-line comparing karyokinetic events in wild-type Oregon R primary spermatocytes and in the two independent P-element-mediated klp67A mutant strains examined. The time is in minutes relative to prometaphase onset. Red bars indicate the reorientation period and anaphase onset is indicated by the blunted ends of each line. Although the mutants enter anaphase with similar timing to the controls, their chromosomes reorient for substantially longer. (B) Selected images from a time-lapse sequence during prometaphase in a klp67A mutant. The black line (00 timepoint) indicates the long axis of the cell. The two homologous dyads comprising the bivalent of interest are pseudo-coloured red and blue. This chromosome continues to reorient for over 22 minutes before moving out of the plane of focus. During this time it makes a few poleward ingressions but generally remains near the spindle equator. Time in upper right of each panel is in seconds relative to an arbitrary start time. Bar, 10 µm.
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