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Fig. 4. Dyads in klp67A mutants segregate at greatly reduced velocities. (A) Selected images from a time-lapse sequence showing a wild-type cell during anaphase. The homologous dyads have been pseudo-coloured and their anaphase movements plotted (B). The average maximal velocity for each of the dyads of interest is indicated. Once wild-type dyads initiate poleward motion it continues uninterrupted until they reach their poles. (A') Selected frames from a time-lapse sequence during anaphase in a klp67A mutant cell. The chromosomes have been coloured as above. (A') Prior to anaphase onset the dyads appear `twisted' relative to one another (–128 second time point). Following disjunction, the dyads in this cell separate and initiate movement to their respective poles. One dyad (blue) travels out of the plane of focus at 
200 seconds. The red dyad begins its poleward migration and then abruptly halts at 152 seconds and remains stationary relative to the spindle pole, and exhibits no further poleward movement before decondensing. (B') Kinetic plot and average maximal velocities for the dyads shown in (A'). Time in seconds relative to anaphase onset is given in the right-hand corner. Bars, 10 µm.
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