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Fig. 5. klp67A mutants form unstable metaphase plates and have merotelically maloriented chromosomes. (A-F) Selected frames from a time-lapse sequence showing metaphase (A) and anaphase (B-F) in a klp67A mutant primary spermatocyte. Prior to anaphase onset, chromosome IV (black arrowhead) travels away from the metaphase plate before moving out of the plane of focus near the lower pole. The adjacent bivalents are abnormally resolved and their individual chromatids are visible. As anaphase ensues, one bivalent (pseudo-coloured) disjoins. Both (red) chromatids of one dyad remain associated and travel to the lower, proximal pole as predicted. The homologous (blue) dyad separates into its two component chromatids, with one chromatid moving towards the expected upper pole and out of focus (D-F), whereas its sister chromatid (white arrowhead) begins to move towards the upper pole (D) then halts before rapidly changing direction and travelling to the lower, incorrect pole (E,F), resulting in aneuploidy. Time is in seconds relative to anaphase onset. Bar in C, 10 µm; F, 2 µm.

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