|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 6. Klp67A associates with pre-anaphase kinetochores and the central spindle during meiosis I. (A,B and D-F) Microtubules are shown in green, Klp67A in red and DNA in blue. During interphase and prophase (A) Klp67A localises exclusively to the nucleus where it remains until prometaphase. At this time, the protein redistributes to the cytoplasm, but is excluded from regions occupied by spindle MTs (B,C). Klp67A is also detected as pairs of opposing spots on the chromosomes (B,C, arrowheads), that interact with presumptive kinetochore fibres (D). Co-localisation studies (G) using anti-CID antibodies to label centromeres or GFP-Polo-tagged outer kinetochore plates, suggest that Klp67A is a new component of pre-anaphase kinetochores (H). Following anaphase onset Klp67A redistributes to an equatorial band on the central spindle (E), where it remains during cytokinesis. During this time Klp67A is still detected in the cytoplasmic pool, and as telophase initiates, in the reforming nuclei (F). Bar in D, 2 µm; F, 10 µm; G, 1 µm.
|
