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Fig. 3. N4WBP5A localizes to cytoplasmic vesicular structures. (A-F) Endogenous N4WBP5A protein detected in M-1 cells by immunofluorescence. (A,D) Localization of N4WBP5A; (B) localization of the Golgi matrix protein, GM130; (C) colocalization of N4WBP5A and GM130; (E) localization of the lysosomal protein, LAMP1 and (F) colocalization of N4WBP5A and LAMP1. (G-L) Ectopically expressed N4WBP5A-myc protein in HeLa cells detected by immunofluorescence. (G,J) Localization of N4WBP5A-myc; (H) localization of the Golgi matrix protein, GM130; (I) colocalization of N4WBP5A-myc and GM130; (K) localization of the late endosomal protein, CD63 and (L) colocalization of N4WBP5A-myc and CD63. Bar, 10 µm.





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