QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. Overexpression of N4WBP5A inhibits receptor-mediated endocytosis. HeLa cells untransfected (A-C) or transfected with N4WBP5A-myc (D-L) were serum starved, cultured with EGF-Alexa Fluor 488 (200 ng/ml, green) for 1 hour at 4°C and EGF uptake was performed at 37°C for up to 25 minutes. Surface-bound EGF-Alexa fluor 488 was stripped from the cells, cells were fixed and permeabilized and immunohistochemistry performed with an anti-myc antibody and anti-mouse Alexa Fluor 568 secondary antibody (red). (A) EGF uptake for 5 minutes; (B) 10 minutes; (C) 20 minutes. (D-F) EGF uptake for 15 minutes. (G-I) EGF uptake for 20 minutes. (J-L) EGF uptake for 25 minutes. (A-C,D,G,J) EGF localization (green, transfected cells outlined). (E,H,K) Transfected cells expressing N4WBP5A (red) and merged images (F,I,L) showing transfected cells and endocytosed EGF Alexa Fluor 488. Bar, 10 µm.

Return to article