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Fig. 7. EGF localizes in early stage endosomes in N4WBP5A transfected cells. EGF uptake was performed in HeLa cells transfected with myc-N4WBP5A for 20 minutes as described in Fig. 5. The line profile tool [LaserPix V4.0 software (Bio-Rad)] was used to measure the fluorescence intensity and size of EGF-containing endosomes in transfected cells (A,B) and untransfected cells (C,D). Graphs showing examples of the fluorescence intensity versus distance in microns measured with the line profile are shown below.





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