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Fig. 8. Overexpression of N4WBP5A does not affect cell-surface levels of the EGFR. HeLa cells were transfected with N4WBP5A or EGFP-N1 as a control and analysed for cell-surface levels of EGFR by indirect immunoflourescence using flow cytometry. Cell-surface EGFR (PE positivity) was measured on untransfected cells, or cells transfected with N4WBP5A-GFP or EGFP-N1. The histogram shows the EGFR levels (PE Log scale) on EGFP-N1+ (open black line) and N4WBP5A-GFP+ (grey filled) cells against relative number of events. The mean PE values for the various populations are shown below.





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