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Fig. 1. Expression of Kin17 mRNA and protein in developmental and adult mouse testes. (A) Total RNA from testes of mice at days 5, 12, 17, 22, 28, 48 and 53 p.p. were subjected to RT-PCR. PCR products were analysed in a 2% agarose gel. The results shown are from two independent experiments done in triplicate. The numerical interpretation of a representative gel is shown. ß Actin RNA was determined as reference. The intensity of the bands was quantified using NIH image software. The ratios Kin17:ß Actin RNA were calculated and plotted. (B,C) Western analysis of KIN17, PCNA and ß ACTIN proteins from extracts of mouse testes. (B) 20 µg of the detergent-extractable protein fraction and (C) 20 µg of the protein remaining in the pellet were loaded per lane. The positive control (PC) includes purified mouse KIN17 protein produced in bacteria.





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