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Fig. 4. Detection of KIN17 protein by immunofluorescence on testicular cell spreads. (A,B) 2-day-old (C) 8-day-old and (D-F) adult mice cells. In neonatal testes, germ cell interphase nuclei are much larger (arrow in A) than somatic nuclei (B). Both germ and somatic interphase nuclei are stained positive (red) for KIN17 all over the nucleoplasm with stronger staining of large intranuclear structures (^* in A and B). Germ and somatic metaphase chromosomes also exhibit different shapes and, in the same preparation, germ cell metaphases are well spread (B) compared to Sertoli cell metaphases (A). In both cell types staining is not detected at pericentromeric heterochromatic regions as shown after counterstaining with DAPI (blue, arrows in B and C). (C) In 8-day-old mice spermatogonia metaphase chromosomes (with premature centromeric region separation as previously described) (Bernardino-Sgherri et al., 2002) exhibit the same staining pattern. At different stages of adult germ cell, chromatin of pachytene (D), metaphase I (E) and metaphase II (F) spermatocytes is stained by anti-KIN17 antibodies, whereas reduced staining is observed at pericentromeric regions (arrows in E and F). Bars, 10 µm.

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