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Fig. 7. KIN17 protein co-purifies with poly(A+) RNA. Extracts from mouse testicular cells were subjected to oligo (dT) chromatography under native conditions. Aliquots from total cell extract (lane 1), flow-through (lane 2), high-salt (lane 3) and low-salt (lane 4) washes and eluate (lane 5) fractions were subjected to SDS-PAGE and analysed by western blot. Previous RNase treatment of cell extracts abolishes KIN17, HUR and DAX-1 fractionation in the eluate (lanes 5 and 6).





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