QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. MIs do not induce myogenin and p21. Asynchronous myoblasts were incubated in control medium or 30 mM BDM or 15 µM ML7 for 24 hours. Myogenin (green) and p21 (red) were simultaneously detected using antibodies. (A) Staining of both early myogenic markers was readily detected in control cells but not in MI-treated cells. Scale bar: 10 µm. (B) The frequency of myogenin and p21 expression (mean ± s.e.m. n=2). The low level of myogenin and p21 expression in growth medium (1), rises after 24 hours in differentiation medium alone (2) or with DMSO vehicle (3). Both markers are suppressed by BDM and ML7 (4,5). (C,D) Induction of quiescence-related protein p27^kip1 after BDM and ML7 treatment. Serum withdrawal caused some induction of p27 as expected (compare 1 with 2 or 3; numbers indicate conditions as in panel B), but MI treatment (4,5) led to increased frequency and intensity of p27 staining (mean ± s.e.m. n=2). Scale bar in C: 25 µm.

Return to article