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Fig. 9. Over-expression of RhoA overrides the effect of BDM on cytoskeletal organization. Asynchronous cultures were transfected as in Fig. 7 and 12 hours later were treated with vehicle (a-f) or 30 mM BDM (g-l) for a further 12 hours, (d-f) phase contrast + DNA stain of a-c. Control GFP cells were well spread with distributed paxillin staining; WT Rho-transfected cells were smaller with increased paxillin-positive focal contacts. Insets in b and c show cells expressing low levels of GFP marker, not visible in the merged images. DNRho transfection suggested a reciprocal effect to WTRho, with dispersed focal contacts. BDM treatment led to cell elongation and reduction of paxillin in GFP transfected cells (g), but did not reverse Rho-mediated cell shrinkage (h), (j-l) phase contrast + DNA stain of g-i. Scale bar: 10 µm.
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