spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. IL-3 stimulates phosphorylation of paxillin tyrosine residues 31 and 118. (A) Baf3 cells were stably transfected with EGFP (vector) or EGFP-conjugated wild-type {alpha}-paxillin or the {alpha}-paxillin Y->F tyrosine phosphorylation mutants 31, 118, 31/118, 40/181, 31/40/118/181, 31/40/181/434/488 and 40/118/181/434/488. After six hours of IL-3 withdrawal, 10 ng/ml IL-3 was resupplied for 10 minutes. Immunoprecipitated paxillin (IP-Pax) was analyzed on western blots with a mixture of anti-phosphotyrosine antibodies 4G10 and PY20 (IB-PY) or anti-GFP antibody (IB-GFP). (B) Immunoprecipitated paxillin (IP:Paxillin) was treated in vitro with non-specific potato acid phosphatase (PAP) or Protein Tyrosine Phosphatase (PTP), which specifically dephosphorylates tyrosine residues and was then analyzed on western blots using anti-paxillin antibody (IB:Paxillin).





Right arrow Return to article