spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. PMA inhibits the IL-3-induced tyrosine phosphorylation of FAK and FAK-binding proteins. Cells were deprived of IL-3 for 6 hours or grown on IL-3 and treated with 4 µM cytochalasin D (CytD), 1µM GF109203X (GF), 100 nM PMA alone or in combination for 30 minutes. (A) After immunoprecipitation with monoclonal anti-FAK antibody (IP:FAK) or non-immune mouse IgG1 (IP:IgG1) in denaturing conditions, lysates were analyzed on western blots with a mixture of anti-phosphotyrosine 4G10 and PY20 antibody (IB:PY), a phosphospecific anti-FAK(pY397) antibody (IB:FAKpY397) or polyclonal anti-FAK antibody (IB:FAK). (B) In another series of experiments, after immunoprecipitation with anti-FAK antibody (IP:FAK) or non-immune mouse IgG1 (IP:IgG1) in non-denaturing conditions lysates were analysed on western blots with phosphotyrosine PY20 antibody (IB:PY), anti-paxillin antibody (IB:paxillin) or anti-FAK antibody (IB:FAK).





Right arrow Return to article