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Fig. 3. Forskolin-induced reduction of cyclin D3 levels is mediated via cAMP. Reh cells were treated with the indicated concentrations of forskolin, IBMX or PGE2 for 2 hours. Following treatment, lysates were prepared, resolved on SDS-PAGE, and then subjected to immunoblotting with cyclin D3 and actin antibodies. The blot shown is the representative of two independent experiments.





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