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Fig. 5. Effect of GSK-3ß inhibition on forskolin-induced degradation of cyclin D3. Reh cells were pretreated with the GSK-3ß inhibitor LiCl (20 mM) for 30 minutes before addition of forskolin (100 µM). Cells were harvested at 2 hours after addition of forskolin and total lysates were analyzed with antibodies against cyclin D3 and actin. The blot shown is the representative of four independent experiments. Lower panel, data shown represent the mean ±s.e. of the four independent experiments. The results in each experiment were quantified using a densitometer and the densitometric values of cyclin D3 were normalized with those of actin.
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