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1 is a guanine nucleotide exchange factor for dynamin-1 and enhances dynamin-1-dependent epidermal growth factor receptor endocytosisFiles in this Data Supplement:
Fig. S1. PRD of dynamin-1 is involved in self-assembly. (A) PC12 cells were transfected with FLAG- and HA-tagged dynamin-1WT and dynamin-1DPRD constructs, immunoprecipitated with anti-FLAG antibody and immunoblotted with anti-HA and anti-FLAG antibody. (B) PC12 cells were transfected with FLAG-tagged dynamin-1PRD and HA-tagged dynamin-1GTPase constructs. Cell lysates were precipitated with GST-fused PLC-g1SH3 proteins. Precipitated proteins were analyzed by immunoblotting with anti-FLAG and anti-HA antibody.
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