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Fig. 5. PLC-{gamma}1's GEF activity potentiates dynamin-1-dependent EGFR endocytosis. PC12 cells transfected with various PLC-{gamma}1 constructs (A) or PLC-{gamma}1 siRNA duplexes (B) were incubated with Rhodamine-conjugated EGF at the indicated times and fixed cells were examined by confocal microscopy. Arrowheads indicate the internalized EGF-EGFR vesicles.





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