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Fig. 5. Production and cell-cycle analysis of cells reconstituted by Daxx wild-type (wt) and truncation mutants. Schematic of constructs is given at the top. Colocalization of reconstituted Daxx wt with HP1{alpha} at condensed chromatin (a, upper and lower nuclei) and with PML at ND10 (d) resemble those of endogenous Daxx. (g) BrdU incorporation followed by labeling of BrdU and Daxx by HA antibody; reconstituted Daxx wt accumulated at heterochromatin only at late S-phase (lower right nucleus). (b) Daxx{delta}C localized with HP1{alpha} at heterochromatin (upper and left nuclei) and is homogeneously dispersed in other cells. (e) Daxx{delta}C does not accumulate at ND10. (h) Daxx{delta}C accumulation at late BrdU incorporation sites (lower right). (c) DaxxC does not accumulate with HP1{alpha} at heterochromatin but maintains exclusive PML/ND10 associated pattern (f) throughout the cell cycle, including cells with a late S-phase BrdU incorporation pattern (i, lower right).





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