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Fig. 1. PfVps4 localizes to the cytosol of P. falciparum trophozoites. (A) Immunoblot of P. falciparum trophozoites with rabbit anti-PfVps4 (lanes 2 and 3) and mouse anti-PfVps4 (lanes 5 and 6). Lanes 1 and 4 are pre-immune serum. Lanes 2 and 3 correspond to two different preparations of P. falciparum, as do lanes 5 and 6. (B) Specific labeling with mouse anti-Vsp4 of the cytosol. ER, endoplasmic reticulum; N, nucleus; Pf, Plasmodium falciparum; DV, digestive vacuole. Bar, 0.15 µm.