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Fig. 3. Subcellular fractionation of P. falciparum and T. gondii expressing GFP-PfVps4. (A) Cell homogenates (T, total cell lysate) prepared from a non-transfected control (3D7) and from P. falciparum stably expressing GFP-PfVps4-WT were fractionated into supernatant (S) and pellet (P) as described in Materials and Methods. Fractions were subsequently analyzed by immunoblot using an affinity purified rabbit anti-GFP antibody. Results are described in the text. (B) The same blot from panel A was stripped and re-analyzed using an affinity purified rabbit anti-PfVps4 antibody. (C) Wild-type T. gondii RH strain, and the RH strain stably expressing GFP-PfVps4-E214Q or GFP-PfVps4 WT were subjected to subcellular fractionation, as described in Materials and Methods. The fractions were analyzed by immunoblot using a rabbit anti-GFP antibody. Results are described in the text.





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