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Fig. 5. Mutant PfVps4 does not co-localize with lipid droplets in T. gondii. (A) Parasite lipid droplets, detected with oil red O (ORO) do not co-localize with mutant PfVps4-positive structures. (B) The distribution of NBD-cholesterol which accumulates in T. gondii is altered by expression of mutant PfVps4, consistent with accumulation of cholesterol in the PfVps4-positive compartments as determined by filipin staining.





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