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Fig. 7. Mutant GFP-PfVps4 generates enlarged, cholesterol-enriched vesicles when expressed in COS cells. Wild type and mutant PfVps4 were transiently expressed in COS cells. GFP fluorescence is illustrated, as is the distribution of cholesterol, detected by staining with filipin. Importantly, immunofluorescence staining of transgenic COS cells revealed precise co-localization between the GFP fluorescence and PfVps4 detected using rabbit or mouse antiserum (not shown).





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