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Fig. 4. Synthetic lethality between vps1 ${Delta}$ and alleles of sla1 with actin defects. (A) The viabilities of various mutants after their SLA1-containing plasmid pTHY918 was lost on a 5-FOA plate: strainsYMC467 (sla1 ${Delta}$ ), YMC468 (sla1 ${Delta}$ mgm1 ${Delta}$ ), YMC469 (sla1 ${Delta}$ dnm1 ${Delta}$ ) and YMC470 (sla1 ${Delta}$ vps1 ${Delta}$ ) grown on SC-Ura plate (left) at 24°C were replica-plated onto a 5-FOA plate (right) and incubated at 24°C for 2 days. (B) The schematic structures of Sla1p and its deletion constructs to be used in the following experiments. (C) The test of the strain YMC470 (sla1 ${Delta}$ vps1 ${Delta}$ ) to lose the SLA1-containing plasmid pTHY918 in the presence of various sla1 deletion constructs: pRS314 (vector, lane 1), pYGS203 (sla1^3rdSH3, lane 2), pTHY1114 (sla1^{all SH3}, lane 3), pTHY1115 (sla1^SR, lane 4) and pTHY1089 (Sla1^FL, lane 5).

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