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Fig. 6. Vps1p is required for the cell cortex and polarized localization of Sla1p. The subcellular localization of Sla1p and actin morphology were altered in vps1 ${Delta}$ and the vps1 GTPase mutants. The genomic copy of SLA1 in strains YMC457 (vps1 ${Delta}$ ), YMC458 (vps1 ${Delta}$ with wild-type VPS1, Vps1^WT) and YMC459-461 (vps1 GTPase mutants, vps1^K42E, vps1^S43N and vps1^G315D, respectively), was tagged with GFP at its C terminus and the resulting strains were incubated at 30°C to the exponential phase followed by actin staining. The actin organization and the subcellular localization of Sla1p from vps1 ${Delta}$ and the vps1 GTPase mutants were visualized under Leica microscopy (bar, 5 µm).

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