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Fig. 2. (A) Immunoblotting with PDGF ß-receptor in 3T3 fibroblasts transiently transfected with LT, small t (st) or expression vector using Fugene cultured with 10% FCS. Lane 1: non-transfected cells, lane 2: 2 µg pcDNA3 vector, lane 3: 1 µg LT and 1 µg pcDNA3 vector, lane 4: 1 µg st and 1 µg pcDNA3 vector. (B) No alteration of PDGF ß-receptor expression in myc-null HO15.19 cells was shown by immunoblotting after transfection with LT, or pcDNA3 vector. Immunoblots with anti-LT and anti-actin antibodies are shown. (C) Expression of PDGF ß-receptor mRNA at 0, 2, 4 and 8 hours after transfection of LT or pcDNA3 vector in 3T3 cells. The RT-PCR products for PDGF ß-receptor, and actin were run on an agarose gel and are also shown as relative ratios of PDGF ß-receptor to actin obtained by densitometric analysis.





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