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Fig. 3. Effects of PDGF ß-receptor promoter activity by LT in 3T3 cells. (A) Schematic illustration of the promoter with relevant restriction enzyme sites. The GC-rich area (white box) and CCAAT motif (black box) are shown. (B) Relative luciferase activity of the SacI/SacI-reporter construct containing CCAAT motif co-transfected with st, LT, and vector alone (Mock). Basic vector without promoter was used as a basal control. Values represent mean promoter activity, and error bars indicate standard deviation of triplicate samples. A representative result of three repeated experiments is shown. The expression levels of LT and st are shown by immunoblotting after transfection. (C) The HindIII/SacI-reporter construct containing CCAAT motif, the MluI/SacI-reporter construct containing CCAAT motif and GC box or the SacI/SacI-construct was co-transfected with Sp1, LT or expression vector alone (mock). The expression of Sp1 is shown by immunoblotting. (D) The SacI/SacI construct was co-transfected with Sp1, LT or vector in the presence of DNNFYA and compared with the effect of LT in the absence of DNNFYA. The expression of NF-YA and DNNFYA is shown by immunoblotting.

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