spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Binding to both p53 and pRb is necessary for LT repression on PDGF ß-promoter activity and the LXCXE Rb-binding mutants enhance the promoter activity via CCAAT motif. (A) 3T3 cells were co-transfected with the SacI/SacI construct together with H42Q, {Delta}434-444, C105G, LTK1 or vector. The expression of all the LT vectors used in the assays is shown by immunoblotting. (B) 3T3 cells were co-transfected with the HindIII/SacI, SacI/SacI or basic reporter construct together with LT, K1, or vector alone. (C) 3T3 cells were co-transfected with the SacI/SacI construct containing wild type or mutated CCAAT motif together with vector alone, LTK1, DNNFYA or DNNFYA and LTK1. Luciferase activity was measured as described above.





Right arrow Return to article