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Fig. 5. LT is unable to repress PDGF ß-receptor promoter activity in (A) Saos-2 cells, (B) HO15.19 cells and (C) Rb null cells. Transfection of Sp1 was used as a positive control. Cells were co-transfected with the SacI/SacI construct together with LT or vector alone examined in 2 different vectors. Expression of LT is shown by immunoblotting. Luciferase activity was measured as described above. (D) Rb null cells express a stable level of PDGF ß-receptor during a cell cycle. Cells were harvested at indicated time points after serum stimulation of starved cells and used for immunoblots with antibodies against PDGF ß-receptor, c-Myc and actin. The lowest panel of 3T3 cells shows non-specific bands.





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