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Fig. 7. LT and p53 bind PDGF ß-receptor promoter in vivo in ST15A cells and Saos-2 cells. (A,B) Chromatin was immunoprecipitated from ST15A cells cultured with anti-LT antibody, anti-p53 antibody or mouse IgG at 33°C (A) and 39°C (B). Immunoprecipitated DNA was purified and analysed by PCR together with whole cell lysate using primers specific for the proximal and distal areas of the PDGF ß-receptor promoter. (C) Total protein was extracted from ST15A cells cultured under both conditions and immunoblotted with anti-LT, anti-p53, and anti-actin antibodies. (D,E) Chromatin was immunoprecipitated from Saos-2 cells (D), and Saos-2-LT cells (E) stably expressing LT with normal rabbit serum, mouse IgG, anti-NF-YB, and anti-LT antibodies described as above. (F) Expression of LT and actin is shown in Saos-2 and Saos-2-LT cells by immunoblotting.
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