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Fig. 5. Nuclear localization of telomeres in cells treated with mating pheromone. h– sxa2{Delta} (TG208) cells (A,B), h– sxa2{Delta} mei2{Delta} (TG263) cells (C-E), and h– sxa2{Delta} spk1{Delta} (TG265) cells (F,G) were treated with the synthetic P-factor, and observed by fluorescence microscopy without fixation. (A,C,D,F) The SPB (red) and telomeres (green) were stained with Sad1-DsRed and Taz1-GFP, respectively. Bright-field images of the same cells are also shown (Phase). Scale bars: 5 µm. (B,E,G) Time course of cell shape alteration (`altered shape') and telomere clustering (`tel clustering') after P-factor treatment. To calculate the percentage of cells with altered shape, 200 cells (B,G) or 130 cells (E) were scored for each time point. To calculate the percentage of cells with telomere clustering, at least 50 cells were scored for each time point (B,E,G).





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