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Fig. 6. Nuclear localization of telomeres in pat1-114 mutant cells. h– pat1-114 (TG292) cells (A-C), h– pat1-114 mei2{Delta} (TG202) cells (D-F), and h– pat1-114 spk1{Delta} (CRLc92) cells (G-I) were shifted to the restrictive temperature to induce ectopic meiosis, and observed by fluorescence microscopy without fixation. (A,D,G) Progression of meiosis after shifting to the restrictive temperature. The number of nuclei per cell was counted for up to 10 hours after induction of meiosis. At least 250 cells were scored for each time point. (B,E,H) The SPB (red) was stained with CFP-Sad1, and telomeres (green) were stained with Taz1-GFP. Bright-field images of the same cells are also shown (Phase). Scale bars: 5 µm. (C,F,I) Time course of telomere clustering after shifting to the restrictive temperature. To calculate the percentage of cells with telomere clustering, at least 50 cells were scored for each time point.





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