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Fig. 8. U73122 (U7) causes reversible regression of cleavage furrows in crane fly primary spermatocytes. (A) Concentration dependence of U7 in blocking constriction. Clear bars, cells in which there was no effect on cleavage. Hatched bars, cells in which cytokinesis was blocked. (B) Graph of a single cell treated with 4 µM U7 (added at the left arrow), which was then washed out with Ringer's solution (right arrow). Note that after addition of U7, the furrow slows, regresses, and then resumes constriction approximately 8 minutes after washout. The dashed line is the computer-generated exponential best-fit curve through the points prior to addition of U7.





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