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Fig. 4. Dynamics of Clp1p-dependent actomyosin ring maintenance upon perturbation of cytokinetic machinery. (A) Cells of the indicated genotypes expressing Rlc1p-GFP were imaged under conditions shown on the left-hand side. The numbers on the top right of each frame indicates the time in minutes. Note that whereas the Rlc1p-GFp rings are maintained for a prolonged period of time, leading to the accomplishment of division septum assembly in wild-type cells upon treatment with a low dose of Lat A (0.2 µM), these rings fragment in similarly treated clp1 ${Delta}$ cells. Note also that SIN mutants display a phenotype similar to that observed in clp1 ${Delta}$ cells treated with a low dose of Lat A. (B) 3D-reconstructions of Rlc1p-GFP expressing cells of the indicated genotype treated with DMSO or 0.2 µM Lat A. Time (t) is indicated in minutes. Wild-type, clp1 ${Delta}$ , and clp1 ${Delta}$ sid1-239 cells were imaged at 32°C. sid1-239 cells were imaged at 36°C.

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