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Fig. 5. SIN mutants are hypersensitive to mild perturbations of the cell division machinery whereas ectopic activation of the SIN compensates for the loss of Clp1p. (AI) cdc14-118, myo2-E1, and myo2-E1 cdc14-118 mutants were streaked to YES plates and assayed for colony formation after 3 days at the semi-permissive temperature of 30°C. (AII, III and IV) cdc14-118, myo2-E1 and myo2-E1 cdc14-118 cells were grown at 24°C to early-log phase, shifted to 30°C for six hours, and then fixed and stained with DAPI and aniline blue to visualize nuclei and division septa, respectively. Scale bar 10 µm. (B) Quantitative data for cells in AII-IV. (C) Cells of the indicated genotypes were grown to early-log phase at 24°C, shifted to 36°C, and then treated with 0.2 µM of Lat A or DMSO (solvent control) for 5 hours. Cells were subsequently fixed and stained with DAPI and aniline blue to visualize nuclei and division septa respectively. Scale bar 10 µm. (D) Quantitative data for cells treated with Lat A in C.
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