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Fig. 10. Effect of inhibitors on the Stx1-B-subunit-mediated clustering of ezrin and vimentin. (A) ACHN cells were preincubated with the inhibitors shown in the figure for three hours. The concentrations of 900 µM for MBD and 100 µM for other inhibitors were used. After treatment with the Stx1-B subunit for 10 minutes, the cells were fixed and stained with anti-ezrin monoclonal antibody (green) followed by counterstaining with DAPI (blue) as in Fig. 2. Results are representative of three independent experiments. The clustering of ezrin was indicated by arrowhead. (B) ACHN cells pre-incubated with inhibitors as in A were treated with the Stx1-B for 30 minutes and stained with anti-vimentin monoclonal antibody (green) as in A.
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