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Fig. 2. Distribution of DRMs during bacterial entry. HeLa cells were infected with C. caviae GPIC for 0 minute (top) or 5 minutes (bottom) before fixation. Actin was visualized with Alexa546-coupled phalloidin (left) and GM1 gangliosides were stained using FITC-cholera toxin (middle). Superimposition of the two stainings is shown (right), clusters of GM1 are indicated (arrows).