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Fig. 6. Effect of the inhibition of Cdc42 and Rac on actin polymerization during bacterial entry. Cells, transfected for 18 hours with dominant negative Cdc42 (A-E) or Rac (F-J), were inoculated with culture medium alone (A,B,F,G) or with FITC-coupled bacteria (C,D,E,H,I,J). Five minutes p.i. the cells were fixed, and transfected cells were identified using anti-myc antibodies (A,C,F,H) while actin was observed using phalloidin (B,D,G,I). E and J show the superimposition of the 3 images collected from the infected samples with the transfected cells (blue), actin (red) and bacteria (green). Both Cdc42N17 and RacN17 were recruited to the sites of actin polymerization, with which bacteria were associated (arrows). Notice that in transfected cells actin patches were larger than in non-transfected cells (arrowheads).
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