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Fig. 1. Immunofluorescence with a rabbit polyclonal antibody against TRF2 (H-300) revealed a nucleolar localization of TRF2 in human MCF-7 cells (A-B) but not in mouse NIH 3T3 cells (C-D). The same antibody was affinity purified with immobilized recombinant TRF2 and used for western blotting of MCF-7 or 3T3 whole cell lysate (E). The affinity purified TRF2 antibody also revealed a nucleolar staining together with several nucleoplasmic dots that are supposed to be telomeres (F-G). Arrows indicate the positions of the nucleolus.





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