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Fig. 7. After treatment with actinomycin D, TRF2 co-localized with UBF in metaphase. The actinomycin D-delayed release of TRF2 from the nucleolus offered the opportunity to visualize its co-localization with UBF. Laser scanning confocal microscopy revealed an adjacent localization between TRF2 and UBF in prophase (A-C), and a complete overlap of both proteins in metaphase (D-F). Arrows indicate NORs where UBF or TRF2 were localized. Images with overlaid DNA stains are presented in A'-F'. C'' and F'' represent magnifications of the framed parts shown in C and F. Fluorescence measurements in C'' and F'' show the co-localization of TRF2 and UBF along the arrowed red lines.





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