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Fig. 8. Sucrose gradient centrifugation revealed high molecular weight TRF2-containing complexes in nucleolar extracts of human cells. Western blotting was performed to detect the presence of TRF2, Ku70 and B23 in the fractions of HeLa nucleolar extracts after ultracentrifugation through a 10 to 40% sucrose density gradient. MNase-sensitive and Ku-containing complexes occurred in fractions 8-16 and MNase-sensitive and B23-complexes showed up in fractions 10-17.





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