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Fig. 2. Cells that lack {alpha}3ß1 do not extend lamellipodia at a wound edge. (A) MK+/+, MK–/– or MK{alpha}3 cells were grown to confluence on collagen-coated glass coverslips, scrape wounded and fixed either immediately after wounding (0 hours) or 2 hours after wounding. Cells were stained for F-actin with TRITC-conjugated phalloidin. (B) MK{alpha}3 cells were stained for ß-actin 2 hours after wounding. The corresponding phase image is shown for each field. Arrowheads point to edges of lamellipodia; arrows point to actin purse strings. Bar, 10 µm.





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