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Fig. 6. Rac1 activation in keratinocytes is dependent on integrin {alpha}3ß1. (A) Cell lysates from MK+/+ cells, MK–/– cells and MK{alpha}3 cells were immunoblotted for Rac1, keratin 14 or {alpha}3 integrin. (B) Representative immunoblot of GST-PAK pull-down assay to detect levels of active Rac1 in MK cells after adhesion to LN-5 ECM for 30 minutes in the absence of exogenous growth factors. (C) Quantitation of Rac1 activity for three separate experiments. Error bars represent s.e.m.





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