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Fig. 1. CGC express LAMP, OBCAM and CEPU-1 on their surface. (A) RT-PCR was carried out on cDNA prepared from E15 cerebellum. Primers were designed to amplify a central region of LAMP and OBCAM occurring in all known isoforms, or to detect CEPU-1 and CEPU-Se, specifically. All three members of the family and both GPI-anchored and secreted forms of CEPU-1 are expressed. (B) Immunofluorescence microscopy of live-stained CGC revealed that LAMP, OBCAM and CEPU-1 are expressed on the cell surface. In all cases, specific punctate staining could be observed. Bar, 25 µm.