QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 3. Interaction between native HTLV-1 envelope proteins and hDlg. (A,B) Jurkat cells were lysed by several freeze/thaw cycles, and membrane and cytosol fractions were separated by centrifugation. The cytosol fraction was submitted to precipitation with either GST alone or GST fused to the CD of HTLV-1 envelope proteins (GST-CD). The precipitated proteins were detected either using a mAb specific for hDlg (A) or a mAb directed against the PDZ domains of MAGUKs (B). (C) Primary HTLV-1 infected CIB T-cells were metabolically labelled for 16 hours and lysed in a 0.5% NP40 buffer. Soluble material was subjected to precipitation with either GST alone or GST fused with the full-length hDlg (GST-hDlg). As a control, viral proteins were immunoprecipitated from cell lysate using sera obtained from HTLV-1 infected patients (IP anti-HTLV-1).

Return to article