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Fig. 2. TLR2 and GM-1 ganglioside FRET measurements before and after LTA stimulation. Energy transfer between TLR2 (Cy3-TLR2) and GM-1 ganglioside (Cy5-cholera toxin) before (A,B) and after (C,D) stimulation by LTA can be detected by the increase in donor fluorescence after acceptor photobleaching. (A,C) Donor (Cy3) image after acceptor photobleaching, (B,D) E image (image resulting from the subtraction of the Cy3 image before photobleaching from the Cy3 image after photobleaching). E as a function of fluorescence, for D:A of 1:1 (squares), 1:2 (closed circles) and 1:4 (open circles) (E). Scale bar: 5 µm.





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