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Fig. 3. Disrupting lipid raft integrity inhibits LPS-mediated cellular activation. CHO/CD14/TLR2 reporter cell line was either not stimulated (A) or stimulated with 100 mg/ml of LTA in 5% HPS for 30 min either in the absence (B) or presence (C) or 60 µg/ml nystatin. The induction of CD25 surface expression was detected with FITC-CD25. Fluorescence was detected using a FACSCalibur (BectonDickinson), counting 10,000 cells per sample. (D) The effect of raft-disrupting drugs on TNF-{alpha} secretion was measured in treated monocytes isolated from the blood of healthy donors with 60 µg/ml nystatin (open circles) prior to stimulation with LTA. Control experiments with cells stimulated with LTA in the absence of raft-disrupting drugs were also performed (closed circles). TNF-{alpha} secretion was measured using ELISA. Each data point represents the mean±s.d. of three independent experiments.





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