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Fig. 2. Indirect immunolocalization of endogenous survivin in LLCPK cells. (A) Survivin accumulates near the centromeres at G2 phase (arrows). (B) In late prophase cells, survivin is between the sister kinetochores (arrows). (C) During prometaphase, survivin changes its morphology as microtubules attach to the kinetochores and chromosomes become bipolarly oriented (the short arrow shows a `relaxed' chromosome and the long arrow denotes a chromosome under tension from both spindle poles). (D) Metaphase. (E) Early anaphase. (F) Anaphase B. The arrowheads denote survivin strands at the central spindle. (G) In telophase cells, survivin concentrates at the cleavage furrow (arrowheads) before final accumulation at the midbody (arrow). Merge images of survivin (red, detected with the monoclonal anti-survivin antibody), kinetochores (green, detected with anti-hCdc20 antibodies or Crest anti-centromere serum), and DNA (blue, stained with DAPI) are shown. (H) The specificity of monoclonal anti-survivin antibody. A single band corresponding to a 16 kDa protein was detected in interphase (I) and mitotic (M, 12 h taxol treatment) HeLa and LLC-PK cells. Bars, 5 µm.





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